Detection of <i>AtRKD4</i> Protein During Induction of Somatic Embryogenesis in <i>Dendrobium lineale</i> Rolfe Transgenic Orchids Carrying <i>35S::GR::AtRKD4</i>

Dendrobium lineale is an Indonesian native orchid from the Spatulata section in Orchidaceae Family. This important because it usually used as a parental plant breeding and predicted to have potential phytochemistry compound. In addition, their natural habitat, this threatened due forest exploitation disaster. Therefore precision mass propagation techniques for need be conducted. Biotechnological approaches through inserting embryo gene such AtRKD4 Arabidopsis thaliana has already been successfully study aims check integration stability of T-DNA harboring 35S::GR::AtRKD4 ten selection transformants detect existence protein after induction by Dexamethasone and/ Thidiazuron. The result showed that were stably integrated into genome D. with molecular weight 28.53 kDa was detected transformant plants being induced 15 µM DEX 3 mgL-1 TDZ 5 days.